Abstract
A standard laboratory procedure with cultures that grow exponentially involves regular dilution (so that the organism is never nutrient limited). When the logarithm of growth is plotted against time, exponential growth then appears as a series of parallel lines. An investigation of the dependence of the growth rate on the ambient conditions may involve switching the culture from one set of conditions to another. In such a situation the culture may require an acclimation period before beginning growth at its revised exponential rate: the resulting plot will start uncertainly, but end with the required straight line. However, if the change in conditions is too great, or the new conditions are close to the limiting conditions that the organism can tolerate, then the culture may suffer complete or partial death: in these cases there may be no exponential growth
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