A PROTOCOL FOR MICRO-PROPAGATION OF Anthurium scherzeranum, SCHOTT PLANTS.

Abstract

This investigation was carried out in 2004 and 2005 at Plant TissueCulture Laboratory, Agricultural Development System Project (ADSP),Ministry of Agriculture, to detect the effect of some media types, different levelsof some cytokinins and growth substances on some growth and rootingparameters of Anthurium scherzeranum, Schott in vitro as well as the influenceof NPK fertilization on plantlets transferred from in vitro to in vivo greenhouseconditions. The obtained results would be briefed in the following : Number ofshoots and leaves were significantly raised by N & N medium, supplementedwith 5.0mg/l for TDZ or BA individually. But, shoot length was significantlyincreased by MS medium free from TDZ or BA supplementation, whereas, freshweight / shoot tip showed varied responses towards the two cytokinins. The MSmedium supplied with TDZ at 2.0 mg/l or 5.0 mg BA/l supplemented to the N N whereas 3.0 mg /L AgNO3 combined with1.0mg GA3 /L significantly resulted the highest record of these two characters.The half strength of MS, supplemented with 0.5 mg IBA /L combination,significantly gave the best growth parameters (shoot length, number of leavesand fresh weight). While root number and length were significantly increasedby 1.0 mg IBA/l added to 1/8 and 1/4 MS strengths, respectively. Plantstransferred from in vitro to in vivo under greenhouse conditions showed asignificant increase in number and length of shoots when fertilized withN:P:K:Mg (19:19:19:32) at rate of 4.0 g /pot, while number of leaves andflowers were significantly improved by 3.0 or 4.0 g /pot N: P: K (19:19:19),consecutively, applied in two equal doses.