A PROTOCOL FOR MICROPROPAGATION OF Chrysanthemum morifolium, RAMAT. PLANTS

Abstract

An in vitro propagation was carried out at Plant Tissue CultureLaboratory, Agricultural Development System Project (ADSP), Ministry ofAgriculture, during the successive seasons of 2004/2005 and 2005/2006, tofind out the favorable methodology for in vitro propagation ofChrysanthemum morifolium, Ramat. The results could be summarized in thefollowing:The largest shoot length and number of leaves /shoot tip or nodeexplants occurred due MS medium supplemented with 0.1 mg /L TDZ, whereasshoot formation (%) was the best when shoot tips or nodes were subculture inWPM medium supplied with 0.1mg /L TDZ, but callus formation favoured the1.0 mg/l TDZ on WPM. Application GA3 at 1.0 mg /L to shoot tips, subcultureon MS medium supplemented with 1.0 mg BA /L., resulted the best shootlength, number of leaves as well as shoot and callus formation. Applying 0.5mg /L TDZ combined with 2.0 mg /L IAA to shoot tip explants gave the morenumber of leaves and shoot formation. While 2.0 mg /L IAA alone on shoottips resulted the longest shoots. The heaviest callus formation occurred onnode explants treated with 0.5 mg TDZ/l.Shoot tips subculture on MS medium supplemented with 1.0 mg/l BAunder light intensity of 1000 lux produced the larger number of leaves andshoot length.Callus formation was the heaviest on node explants under 1000 lux lightintensity. The largest number of leaves and shoot length occurred on shoottips subculture on MS medium supplied with 1.0 mg /L BA under 240C.But,callus formation was the best on node explants under 17 0C. Addingsucrose to MS medium supplemented with 1.0 mg /L BA was the bestcompared to fructose and glucose as sources of carbon in producing shootlength, number of leaves and callus formation. The highest values ofcharacters occurred due to 20 g /L sucrose combined with shoot tip explants.Shoot length and number of leaves/ shoot tip or node explants were the largestwhen treated with 1.0 mg /L BA, combined with 15 g. /L sucrose. Whereasnumber of shoots on shoot tips, or node explants was the highest at 1.0 mg /LBA with 10 g /L sucrose. The 1.0 mg /L IAA combined with 15 g /L sucrose ledto the heaviest callus formation. While root length was the longest by 0.1mg /LBA combined with sucrose at 10 g /L. MS medium at 1/2 strength resulted thelargest number of roots, root length and root formation, While full strength ofMS failed to induce roots .Reducing MS strength caused concomitant decreasein the growth; characters shoot length and number of leaves. MS salts mediumat full strength gave the largest growth characters.