A Preliminary Study: Troponin T and Reg3β in Children with Left-to-Right Shunt Congenital Heart Disease with Heart Failure

Abstract

Background: Congenital heart disease (CHD) cause heart failure and myocardial injury. Troponin in the heart is a biomarker of myocardial injury in adults and children. Studies that examine troponin T and Reg3β in children with left-to-right shunt CHD with heart failure are still limited. Objective: This study aims to analyse the troponin T and Reg3β in children with left-to-right shunt CHD with heart failure compared to children without heart failure. Method: This study was a case control study of children with left-to-right shunt CHD with heart failure and children with left-to-right shunt CHD without heart failure performed with non-random sampling consecutive techniques at the Dr. Soetomo General Hospital, Surabaya in April–June 2019. The diagnosis of left-to-right shunt CHD was determined based on echocardiographic examination. All subjects with left-to-right shunt CHD were evaluated using the Paediatric Heart Failure Score. Troponin T examination was carried out using a one-dimensional electrophoresis technique, which is 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Reg3β examination was carried out by the ELISA method. Data analysis was performed with an independent sample t test using the SPSS. Results: This study involved 11 children, consisting of 7 children with left-to-right shunt CHD with heart failure and 4 children with left-to-right shunt CHD without heart failure. Most children (72.7%) were female, 3 children (27.3%) were > 5 years old, 5 children (45.4%) were 5–10 years old and 3 children (27.3%) were > 10 years old. There was a significant increase in the Troponin T and Reg3β in children with left-to-right shunt CHD with heart failure as compared to children without heart failure. Conclusion: Troponin T and Reg3β can be used as biomarkers in children with left-to-right shunt CHD with heart failure.