Abstract
The findings indicate that mosaicism does occur in triploids and especially in triploid hybrids. Immature diploid and triploid salmon were obtained from the University of Alaska – National Marine Fisheries Service. These were fixed in 10% buffered formalin and blocks taken from various tissues including kidney, liver, spleen, and gonads. The paraffin blocks were sectioned at 4 μm and stained with hematoxylin and eosin for histologic evaluation and to quantitate nuclear DNA. The slides for DNA evaluation were stained using the Cell Analysis Systems, Inc. (CAS, Elmhurst, IL) kit following Feulgen hydrolysis. The Cell Analysis Systems, Inc. (Model 200) computer-assisted image analyzer was used for DNA quantitation. An attempt was made to evaluate the frequency of mosaicism, ploidy variation in somatic and gonadal tissues of triploids and hybrids as compared with normal diploids. Hepatic ploidy variation, normal in adult animals, was not present in these specimens. However, in gonads there was evidence of increased mosaicism among the triploids. Because of mosaicism in gonadal tissue, sterility of triploids cannot be presumed with certainty at this stage. Further studies are planned to examine this and other questions raised by these results.
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