Abstract
A pre-anodized inlaying ultrathin carbon paste electrode (PAIUCPE) was prepared by electrochemical pretreatment. The scanning electron microscope (SEM) was applied to characterize the surface morphology of PAIUCPE and the performance of the electrode was characterized by cyclic voltammetry (CV). The results indicated that PAIUCPE displayed excellent electrocatalysis for the oxidation of uric acid (UA) and folic acid (FA). The separated extent between the two oxidation peaks of UA and FA was 324mV, which was enough for the simultaneous detection. In 0.10mol/L PBS (pH 6.00), the linear scan voltammetry (LSV) response of UA and FA increased linearly with the concentration in the range of 4.0×10−6–3.5×10−4mol/L and 3.0×10−6–2.0×10−4mol/L with the detection limits of 1.1×10−7mol/L and 1.5×10−7mol/L, respectively. It was successfully used to determine UA and FA in human urine simultaneously.
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