A potential role of nettle (Urtica dioica) extract on growth, biochemical changes and reproductive performance of convict cichlid (Amatitlania nigrofasciata).

Abstract

This study was carried out to investigate the effects of methanolic extract of nettle (Urtica dioica) on growth, reproduction, biochemical and immunological parameters of female convict cichlid (Amatitlania nigrofasciata). For this purpose, 54 fish were distributed in 3 treatments included: without supplementation (control), 0.1g (NE0.1), and 0.5g (NE0.5) nettle extract per kilogram feed over 56days. The highest final weight (4.2 ± 0.1g), weight gain (2.8 ± 0.1g), and specific growth rate (2.0 ± 0.0% day-1) were recorded in NE0.1 group. Higher and lower feed conversion ratio were obtained in control and NE0.1 treatments, respectively. Hepatosomatic and viscerosomatic indices in NE0.1 treatment were significantly lower compared with control treatment. Fish fed NE0.1 showed significantly lower glucose (55.2 ± 6.5mgg-1), cholesterol (28.4 ± 3.3mgg-1), and triglyceride (211.5 ± 39.0mgg-1) levels. Total protein (36.3 ± 3.4mgg-1) and albumin (2.7 ± 0.1mgg-1) showed a marked increase in NE0.1 treatment. Same trend was observed in C3, C4, and IgM concentrations. NE0.1 showed the highest number of eggs per female (183.7 ± 10.2), hatching rate (97 ± 0.7%), and larval survival rate at 3 days post hatch (86.3 ± 0.6%) compared with the other treatments. These findings indicated that 0.1g methanolic extract of nettle kg feed-1 could enhance growth, improve metabolic, and immune function of convict cichlid. Moreover, this study confirmed that appropriate dose of nettle can positively promote reproductive performance which makes it as a valuable and cost-effective herb in aquaculture industry.