Abstract
BackgroundPolycystic ovary syndrome (PCOS) is one of the most prevalent endocrinopathies in women during the reproductive age. Herbal medicines are used increasingly alone or in supplement with chemical medicines for the treatment of different diseases and dysfunctions. This study was aimed to evaluate the effects of lutein and nettle (Urtica dioica) extract on the biochemical parameters and the reproductive function in the PCOS model of mice.MethodsFollowing the induction of PCOS by dehydroepiandrosterone (DHEA), the mice (n = 98) were randomly assigned into seven groups, each consisting of fourteen mice; the groups were included control group (received solvent), PCOS group (received 6 mg/100 g B.W/day IP, DHEA for 21 days), PCOS+ Nettle extract (200 and 400 mg/kg), PCOS+ Lutein (125 and 250 mg/kg), and PCOS+ NL (200 mg/kg nettle extract and 125 mg/kg lutein). The nettle extract and lutein were administrated using gavage for 30 consecutive days after PCOS induction. Malondialdehyde (MDA), total antioxidant capacity (TAC), and estrogen were measured in serum, ovary, and uterus samples by the ELISA method. The total number of oocytes, oocyte quality, fertilization rate, 2-cell blastocyst, and arrested embryos (type I, type II, and type III) were also investigated.ResultsA combination treatment of the nettle and lutein produced the lowest concentration of MDA in comparison to other groups which affected by the PCOS. The lowest level of TAC was observed in the PCOS group without treatment. The number of oocytes, oocyte quality, fertilization rate, and 2-cell blastocyst were significantly higher in the control group, but the lowest values were observed in the PCOS group without any treatment.ConclusionsThe most favorable findings include improving antioxidant capacity, oocyte and embryo quality were observed in the PCOS+ 125 L group.
Highlights
Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrinopathies in women during the reproductive age
Vaginal cytology examination revealed the PCOS mice were interrupted in metestrus phase and the control group was in the metestrus stage in spite of regular estrous cycle at days 21 and 51
Ovary estradiol-17β concentration: The lowest estradiol-17β levels were observed in the control group (44.00 ± 1.82 pg/ml) while the highest level was recorded in the PCOS group (83.75 ± 2.21 pg/ml)
Summary
Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrinopathies in women during the reproductive age. Polycystic ovary syndrome (PCOS) has been reported as one of the most prevalent endocrinopathies in women during the reproductive age [1]. PCOS is known as one of the metabolic disorders with reproductive side effects [2]. PCOS has been associated with three phenotype properties including hyperandrogenism, polycystic ovaries, and ovulatory dysfunction [5]. The circulating markers of oxidative stress were abnormal in women with PCOS. It means that oxidative stress could have a role in the pathophysiology of PCOS [12] Several factors such as formation of reactive oxygen species (ROS) are involved in low-quality of embryos following in vitro culture. The formation of free radicals exceeds the embryos’ antioxidant capacity during in vitro culture of embryo in mammals; different exogenous antioxidants are used to overcome the imbalance oxidative conditions [14]
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