Abstract
A paper strip biosensor was fabricated through the combination of horseradish peroxidase and glucose oxidase with 4-aminoantipyrine and phenol in order to produce a pink color. The color intensity was measured with a smartphone for quantitative analysis. To achieve highest paper strip biosensor performance, the concentrations of glucose oxidase, horseradish peroxidase, phenol, 4-aminoantypyrine, pH, and reaction time were studied. Under optimizations, the linear range of the glucose was 0.5-10 mM, and the detection limit was 0.17 mM. The detection of glucose was achieved within 7 min. The paper strip biosensor was successfully applied to the measurement of glucose in rice samples. The paper strip biosensor exhibits considerable promise as a portable instrument for the detection of glucose in rice and is also applicable to other types of samples. This research, nevertheless, necessitates controlled experimental conditions. Due to the fact that it impacts enzymatic activity.
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