Abstract
A portable, disposable, low-cost, metal-free voltammetric sensor was designed to measure uric acid (UA) in serum samples. The sensor utilizes a nitrogen (N) and sulfur (S) co-doped conductive acetylene black (N, S-CAB) composite, synthesized by a hydrothermal process. N, S-CAB and carbon nanotubes (CNTs) were dispersed via sonication to form a binary nanocomposite, which fosters the electrochemical oxidation of UA. As indicated by EIS, a binary complex formed by hybridizing N, S-CAB and CNTs synergistically enhanced the electron transfer ability. The structural properties of the fabricated sensor were investigated using SEM, EDX, FTIR, and Raman spectroscopy. The electrochemical oxidative behavior of UA was studied using CV, EIS, and LSV measurements. Under optimal conditions, the UA sensor exhibited two linear ranges (0.8–100.0 and 200.0–1000.0 μM) with a limit of detection (LOD) of 0.49 μM. The developed sensor successfully detected UA in the presence of coexisting dopamine (DA) and ascorbic acid (AA), indicating the effective electrocatalytic performance of the designed sensor. The practical application of the sensor was assessed in blood serum samples with satisfactory recoveries, which were consistent with the clinical methods. Overall, the sensor offers a reliable approach for the on-site analysis of UA, DA, and AA, characterized by excellent reproducibility, repeatability, anti-interference ability, and storage stability.
Published Version
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