A population global optimization algorithm to solve the image alignment problem in electron crystallography

Abstract

Knowledge of the structure of biological specimens is critical to understanding their function. Electron crystallography is an electron microscopy (EM) approach that derives the 3D structure of specimens at high-resolution, even at atomic detail. Prior to the tomographic reconstruction, the images taken from the microscope have to be properly aligned. Traditional alignment methods in electron crystallography are based on a phase residual function to be minimized by inefficient exhaustive search procedures. This work addresses this minimization problem from an evolutionary perspective. Universal Evolutionary Global Optimizer (UEGO), an evolutionary multimodal optimization algorithm, has been applied and evaluated for the task of image alignment in this field. UEGO has turned out to be a promising technique alternative to the standard methodology. The alignments found out by UEGO show high levels of accuracy, while reducing the number of function evaluations by a significant factor with respect to the standard method.