Abstract
Insuline-like growth factor binding protein 2 (IGFBP2) is one of the principal binding proteins that has biological functions involved in growth, development, and differentiation. Selection for rapid growth based on molecular Marker Assisted Selection is required to increase production performance. The present study was designed to analyze the associations of IGFBP2 gene polymorphisms with chicken growth and body composition traits. Kampong chicken, a native chicken in Indonesia, is slow-growing chicken. A total of 59 males were used in the current study. Growth and body composition were measured in 24 wk of age. Primers for intron 2 region were designed from genomic chicken sequence. A c.1032C>T SNP of the IGFBP2 gene intron 2 region was detected and PCR-RFLP method was then used to genotype Kampong chicken population. The result showed that IGFBP2 polymorphism was significantly associated with body, carcass, breast, breast muscle, pectoralis minor, leg, and wings weight in Kampong chicken population (P < 0.05). The research suggests that the IGFBP2 gene could be a candidate gene that affects growth and body composition traits in chicken.
Highlights
IntroductionA native chicken and one of genetic resources in Indonesia, has important roles in suplying egg and meat
Kampong chicken, a native chicken and one of genetic resources in Indonesia, has important roles in suplying egg and meat
The model was fitted with the genotype of Insuline-like growth factor binding protein 2 (IGFBP2) gene intron 2 (G: 3 levels: CC, CT, TT) as fixed effects, as follows, Y = μ + G + e; Where Y is the dependent variable for trait measured in the population, ì is the overall population mean for traits, G is the fixed effects, Polymorphism in the Intron 2 Region of Chicken IGFBP2 Gene
Summary
A native chicken and one of genetic resources in Indonesia, has important roles in suplying egg and meat. The selection for rapid growth in meattype chicken is required to increase production performance. IGFbinding proteins extend the half-life of the IGFs and have shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They change the interaction of IGFs with their cell surface receptors (Chesik et al, 2007). There are 7 IGFBP that have been identified succesfully, and they were named IGFBP1 to IGFBP7 (Shimasaki and Ling, 1991; Oh et al, 1996) In mammals, these IGFBPs play a role in modulation of IGFs action by preventing insulin-like effects, regulation of halflife of IGFs in circulation, association with IGFs as carrier proteins and redistribution of IGFs between tissues and extracellular fluids (Kim et al, 2010). IGFBP2 are detected by real-time Polymorphism Chain Reaction (rtPCR) technique in broiler chicken (Leach et al, 2006; Lu et al, 2010)
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