Abstract
Objective To induce Graves' disease in rhesus monkeys,which would exhibit greater similarity to Graves' patients than previous rodent models.Methods Adult female rhesus macaques were injected intramuscularly with 6× 1010 particles adenovirus expressing the A-subunit of TSHR (A-sub-Ad) or EGFP-Ad five times by three weeks intervals,and sera were prepared from blood samples collected at several time points during the immunization regimen.The animals were euthanized 4 weeks after the final injection to obtain blood,spleen cells,thyroid glands and other organs.Results After final immunization,the monkeys injected with A-sub-Ad showed characteristic of Graves' disease.Compared to controls,all the test monkeys had significant higher TRAb levels,half of them had increased TT4 and FT4,and 75％ developed goiter.Conclusion The non-human primate model of Graves' disease was successfully established,which would provide an ideal tool for further understanding of the pathogenesis of Graves' disease in human. Key words: Thyrotropin receptor; A-subunit; Graves' disease; Animal model; Rhesus monkeys
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
