Abstract
Objective To evaluate the therapeutic effect of bone marrow stromal cell transplantation on Parkinson＇s disease （PD） in rhesus monkeys. Methods Experimental PD was induced in rhesus monkeys by stereotatie surgery to destroy the unilateral substantia nigra. Human bone marrow stromal ceils were injected stereotaxically into the 9 target areas in the brain of the monkeys, after which behavioral test was performed and the alteration in the dopamine transporter （DAT） in the substantia nigra was examined using single-photon emission computerized tomography （SPECT）. Immunofluorescence assay was employed to examine the TH- and BrdU-positive cells in the injured and contralateral substantia nigra. Results All the monkeys showed progressive deterioration of the PD symptoms after the cell transplantation. SPECT revealed no significant changes in DAT in the bilateral substantia nigra, but immunoflurescence assay showed that a proportion as high as 25% of the transplanted BMSCs differentiated into dopaminergic neurons. Conclusion Human BMSCs can not serve as the donor cells for treatment of PD in rhesus monkeys. Key words: Parkinson＇s disease; Bone marrow stromal ceils; Transplantation
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