Abstract
AbstractThe mechanism of I700 decay, representing an early event in the phytochrome Pr→ Pfr phototransformation, was reanalyzed in the microsecond range by conventional laser flash photolysis as well as by two‐laser/two‐color flash photolysis. Three kinetic models that might describe the I700 decay mechanism following Pr excitation were considered: a parallel, a sequential, and an equilibrium model. These models were used to mathematically simulate both the one‐ and two‐laser flash experiments in an effort to select the model best describing the I700 decay. The sequential model could be excluded already on the basis of the one‐laser flash photolysis results alone. Discussion of the two‐laser/two‐color flash rcsults in the context of the equilibrium and the parallel models is presented.
Published Version
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