Abstract
As one of the most widespread raptors in Asia, Crested Serpent Eagle (Spilornis cheela) are genetically varied. However, due to the limited studies on genetic aspects, the genetic difference based its geographic distribution are difficult to define. A thorough phylogenetic analysis is needed to create a coherent taxonomic definition for the sake of conservation; starts with phylogenetic analysis based on cytochrome-c oxydase subunit I (COI) gene as the standardized genetic marker to define species. This research was aimed to resolve within species phylogenetic diversity and to explain the relationship between species based on COI gene. Total DNA was isolated from blood samples. The BLAST confirmed PCR amplified COI gene fragments were visualized on 0.8 agarose gel. Sequencing was carried out by FirstBase Laboratory Malaysia. Phylogenetic analysis was done using AMOVA and MEGA7 software in Neighbor Joining method with Kimura-2 parameter. Reconstructed phylogenetic tree confirmed the divergence between three samples and another S. cheela from other regions respective to their geographic distributions. There was 0,014 genetic distance between samples and S. cheela and 0,015 between S. cheela and other species among genus Spilornis. This research also revealed that S. cheela and S. holospilus were not significantly varied.
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