Abstract
A novel photoelectrochemical biosensor for quantitative detection of galactose was successfully developed by immobilizing galactose oxidase on TiO2 nanorod arrays modified F-doped tin oxide (FTO) electrode. Specifically, the direct electron transfer to galactose oxidase was achieved. TiO2 nanorod arrays directly grew on FTO electrode from peroxotitanium complex precursor under low-temperature and normal-pressure conditions. The large surface area and good uniformity of TiO2 nanorod arrays facilitated direct electron transfer between the electrode surface and galactose oxidase. The photocurrent spectra of as-prepared biosensor showed an obvious photocurrent response under UV light irradiation. The generated photocurrent of the stable platform was significantly enhanced after the addition of galactose in solution and the photocurrent intensity possessed linear relationship with the galactose concentration. Moreover, CaCl2, uric acid and ascorbic acid had no interference with the detection of galactose on the working electrode. This sensor can be reused and measure the concentration of galactose in lactose-free milk.
Published Version
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