Abstract
In this study, a pH-response polyacrylate amphiphilic copolymer PMDB was used as the carrier to immobilize laccase from trametes versicolor. Under optimized conditions, the activity recovery of the immobilized laccase is 71.90%. The infrared spectroscopy results indicate laccase is successfully immobilized on the polymer PMDB. Also the properties of the immobilized laccase were studied. The recovery of the immobilized laccase is 96.8% (wt%) by adjusting the pH of the immobilized laccase solution to 3.3. And the immobilized laccase remains 78.13% of the initial activity after 5 batches reactions. Compare to the free laccase, the stabilities like thermal and storage stabilities of the immobilized laccase are enhanced. Moreover, the relative activity of immobilized laccase is nearly 110% when the concentration of urea in the solution is 0.02 M. The work in this manuscript can provide valuable references for enzyme immobilization.
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