A pattern recognition receptor C-type lectin TcCTL14 contributes to immune response and development in the red flour beetle, Tribolium castaneum.

Abstract

Evidence is accumulating that pattern recognition receptor (PRR) C-type lectins (CTL) play essential roles in recognition of pathogens. TcCTL14 (accession no. TC00871) contains the most domains among all CTL of Tribolium castaneum. Yet the biological function of TcCTL14 remains unclear. In this study, TcCTL14 exhibiting typical motif and domain of CTL was cloned from T.castaneum. The expression pattern analysis showed that TcCTL14 was highly expressed in late pupae and central nervous system, and was upregulated after treatment with Escherichia coli and Staphylococcus aureus, respectively. Analysis of binding affinity revealed that recombinant TcCTL14 not only could bind to lipopolysaccharide and peptidoglycan in a dose-dependent fashion, but possibly could bind to and agglutinate different bacteria in a Ca2+ -dependent fashion. Knockdown of TcCTL14 before injection with bacteria led to the downregulation of nuclear factor-κB transcription factors of Toll/IMD and 4 antimicrobial peptides. Knockdown of TcCTL14 also caused suppressed metamorphosis, reduced fecundity, and delayed embryogenesis of T.castaneum. Further observation discovered that knockdown of TcCTL14 inhibited the development of ovaries and embryos. The detection of signaling pathways revealed that TcCTL14 may be involved in metamorphosis and fecundity by impacting 20-hydroxyecdysone and vitellogenin, respectively. Overall, these results indicate that TcCTL14 may contribute to immune response by agglutination or regulating the expression of antimicrobial peptides by the Toll/IMD pathway, and is required for T.castaneum development including metamorphosis, fecundity, and embryogenesis. These findings will improve the functional cognition of PRR CTL in insects and provide the new strategy for pest control.