Abstract
This paper reports the cloning of a cDNA (Vupat 1) expressed in Vigna unguiculata leaves coding for a protein with 48% sequence homology to patatin, the major protein from potato tuber which has lipolytic acylhydrolase activity. Two cultivars differing in drought tolerance were ex-amined in Northern-blot analyses. Expression of Vupat 1 is stimulated by drought stress, especially in the drought-sensitive cultivar. Vupat 1 was expressed in the baculovirus system as a fusion protein secreted in the culture medium. The recombinant protein displays lipolytic activity towards monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerols.
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