A patatin-like protein with galactolipase activity is induced by drought stress in Vigna unguiculata leaves

Abstract

This paper reports the cloning of a cDNA (Vupat1) expressed in Vigna unguiculata leaves coding for a protein with 48% sequence homology to patatin, the major protein from potato tuber which has lipolytic acylhydrolase activity. Two cultivars differing in drought tolerance were examined in Northern-blot analyses. Expression of Vupat1 is stimulated by drought stress, especially in the drought-sensitive cultivar. Vupat1 was expressed in the baculovirus system as a fusion protein secreted in the culture medium. The recombinant protein displays lipolytic activity towards monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerols.