Abstract
A particulate fraction of adult rat brain (sucrose buoyant density 1.24 gm/ml) catalyzed the incorporation of [ 3H]dTTP into an acid-insoluble product in an endogenously templated reaction sensitive to ribonuclease pretreatment. Upon fractionation, this activity was identified in the cerebellum, pons, frontal lobes and base. The DNA polymerase present in these brain fractions exhibited a strong preference for the synthetic template dT 12–18·poly rA rather than dT 12–18·poly dA; dT 10 was completely inactive. Purification and equilibrium Cs 2SO 4 gradient centrifugation of the [ 3H]DNA product-endogenous template complex suggested that RNA was serving as primer for endogenous DNA synthesis.
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