Abstract
In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization. The data are supplemental to our original research article describing detailed associations of pM-ECM as a clinically relevant scale, three-dimensional scaffold with a vascular network template for tissue-specific regeneration, “Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template” Zhang et al. (2016) [1].
Highlights
In this dataset, we depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix using vascular corrosion casting
● This data will be helpful for the research community that harvests and decellularizes porcine rectus abdominis (RA) via the vasculature perfusion for regenerative medicine and tissue engineering
The external iliac artery, the femoral artery and the pudendal epigastricus trunk, which is formed by inferior epigastric artery, epigastric caudalis superficialis artery and pudenda external artery, together with their accompanying veins were carefully isolated and skeletonized, after pulling the peritoneum cranially with partial exposure of the retroperitoneal space (Fig. 3A)
Summary
Table, figure Canon 550D, surgical harvest, perfusion decellularization, vascular corrosion casting Raw, processed No pre-treatment. ● This data will be helpful for the research community that harvests and decellularizes porcine rectus abdominis (RA) via the vasculature perfusion for regenerative medicine and tissue engineering. ● This data allows the scientific community to prepare RA pM-ECM on a clinically relevant scale with similar shape and huge volume to human skeletal muscle. ● This data elucidates the unique retained vascular trees in pM-ECM using corrosion casting. ● This data provides the research community to gain more insight about perfusion decellularization for biologic graft from solid organ with higher density.
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