A novel thermophilic histone with promising gene transfection efficiency

Abstract

concerns with respect to stimulus selection to trigger shape recovery and the integrated functions. Direct heating has been themost common stimulus to use, however it is not always practical for in vivo applications. High-intensity focused ultrasound has been a subject of interest for decades in biomedical research [2]. It may also have a prominent selective heating effect on a particular locationwithminimal effect on surrounding tissue, whichmakes on-demanddelivery of drugs possible. This work aims to evaluate the feasibility of using an SMP carrier based on chitosan modified poly(lactic-co-glycolic acid) (CTSPLGA) microspheres for controlled drug release upon exposure to ultrasound. Fluorescein isothiocyanate labeled lysozyme (FITC-Lyz) is entrapped within the microspheres by the emulsion evaporation method (Fig. 1a). A cylindrical rodwas subsequently fabricated through hot-pressing the microspheres into a mold (Fig. 1b). Results from release assays (Fig. 1c) indicate that the released amount of Lyz increases with the duration and applied power of ultrasound irradiation. The shape recovery process of the drug loaded SMP rod can be controlled simultaneously. Our current demonstration will pave the way for developing even more sophisticated and multifunctional ultrasound-responsive scaffolds and implants for translational biomedical research and applications.