Abstract
In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode immobilized with tetrahydrocannabinol (THC) antibody derived from Balb/c mice was developed. To increase the fixed quantity of antibodies and electrochemical signals, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, a transmission electron microscope (TEM) was used to characterize the nanogold solution. To evaluate the quality of the immunosensor, the amperometric I-t curve method was applied to determine the THC in PBS. The results showed that the response current had a good linear correlation with the THC concentration range from 0.01~103 ng/mL with a correlation coefficient of 0.9986. The lowest detection limit for THC was 3.3 pg/mL (S/N = 3). Moreover, it was validated with high sensitivity and reproducibility. Apparently, the immunosensor may be a very useful tool for monitoring the THC.
Highlights
A Novel Tetrahydrocannabinol Electrochemical NanoImmunosensor Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles
There are over 70 kinds of cannabinoids separated from dry matters of hemp and fresh leaves of hemp, in which the amount of tetrahydrocannabinol (THC) is the highest
Nanomaterials like Gold Nanoparticles (GNPs) possess good conductivity, larger surface area, and good biocompatibility, which is helpful in the immobilization of an increased number of biomolecules to help reach the goal of amplifying electrochemical signals
Summary
Immunosensor Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China. College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, China. Received: 8 September 2016; Accepted: 12 October 2016; Published: 17 October 2016
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