A Novel Stimulation and impedance sensing Setup for Dielectrophoresis based Microfluidic Platform

Abstract

The Dielectrophoresis force delivers an effective and appropriate way to control particles, especially for cell-selective manipulation and cell separation. It has been applied in cell sorting and drug development. Dielectrophoresis force has been applied in the cell trapping process, cell alignment, separation, and isolation of different sized particles, which are applied in a variety of biological applications. Micro electric impedance spectroscopy (µEIS) are very tiny devices that use fluid as a working medium in conjunction with biological cells to extract different electrical parameters. Many advantages can be provided by using these tiny microfluidic devices, such as portability, disposable, and high accuracy.Polystyrene beads are extensively utilized as reference particles to show the efficiency and to validate quantitatively the detectionof Dielectrophoresissystems. In this work, two different forms of trapping dielectrophoresis force combined with a new strategy of impedance extraction are presented and discussed. The study aims to enhance the microfluidic system to differentiate between cells and particles with low conductivity and different sizes. The analysis technique is combined with impedance spectroscopy in a single microfluidic chip that not only enables efficient trapping of cells but also enhances the electric impedance of cells in a label-free and non-invasive using different sizes of Polystyrene Particles.