Abstract

A ratiometric fluorescent thermometer (RPE/ZIF-8) is prepared by embedding red-emitting fluorescent proteins (R-phycoerythrin, RPE) into zeolitic imidazolate frameworks (ZIF-8) through a solid-confinement process. After encapsulated into ZIF-8 crystals, RPE proteins are uniformly distributed in the composite membrane. The resulting RPE/ZIF-8 thin film has three fluorescence emission peaks at 520, 605 and 650 nm, whose fluorescent intensity will decrease regularly as environment temperature increasing. This fluorescent thermometer has wide measurement range and excellent sensitivity, which can be applied for some low temperature detections after assembled into a portable device.

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