Abstract
In this article, we report for the first time the use of a dual-emission carbon dots (CDs) with orange-yellow fluorescence for the detection of l-glutamic acid (L-Glu). The CDs was synthesized through a facile strategy of one-pot hydrothermal route using o-phenylenediamine (oPD) and oxalic acid. The CDs exhibit two fluorescence emission peaks around 453nm and 560nm when the excitation wavelength is at 390nm. In the existence of L-Glu the fluorescence at 560nm was decreased, whereas the fluorescence at 453nm was constant. The fluorescence intensity ratio at 560nm and 453nm (F560/F453) expressed two great linear relationships in the L-Glu concentration range from 0 to 200μM and 200-400μM, respectively, with a detection limit (LOD) of about 0.085μM. In addition, it was used to analyze L-Glu in fetal bovine serum samples successfully, which recoveries were ranging from 97.07 to 103.7%. Those results demonstrate CDs can be further explored in biomedicine studies.
Published Version
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