Abstract
A novel optical fiber glucose biosensor based on fluorescent carbon quantum dots (CQDs)-glucose oxidase (GOD)/cellulose acetate (CA) complex sensitive film was fabricated, in which the dip-coating method was adopted to immobilize the CQDs-GOD/CA complex sensitive film onto the end face of the optical fiber. The surface morphology, microstructure and optical performances of the sensitive film were characterized by field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Zeiss Axiovert 25 inverted microscope, Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectrophotometer and fluorescence spectrophotometer, respectively. The developed fiber-optic biosensor exhibits high sensitivity and repeatability for continuous online detection of low concentration glucose, allowing visualization of real-time glucose fluctuations over a period of time. The change ratios in fluorescence intensity of the biosensor are linear with glucose concentration in various ranges including micromole and nanomole levels, and the relationship between relative fluorescence intensity ratio and glucose concentration complies well with the modified Stern-Volmer equation in the range of 10–200 μmol/L with the detection limit of 6.43 μM, and in the range of 10–100 nmol/L with the detection limit of 25.79 nM, respectively.
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