A novel murine excisional wound model with advanced silicone ring implantation

Abstract

Objective Provide a novel,reproducible,reliable murine excisonal wound model by implanting a silicone ring subcutaneously in advance of dorsal wound creation.Methods A silicone ring was implanted subcutaneously in one slide of the mouse dorsum one week before further operation.In sutured group (n =12),silicone rings were sutured with surrounding normal skin,whereas rings remained untouched in unsutured group ( n =12).8 mm full-thickness excisional wounds were created both in the center of silicon ring and on the other side of midline (control group,n =12).Wounds were observed and evaluated,epidermal thickness,collagen architecture and deposition,cell proliferation and angiogenesis were assessed.Results Compared to control group or unsutured group,wound healing in sutured group was significantly prolonged ( P ＜ 0.05 ).Wound re-epithelialization and collagen deposition were found improved in fixed group( P ＜0.05 ).No significant difference was found between control group and unfixed group(P ＞0.05).Conclusion The excisional wound model with advanced silicone ring implantation can mimic and reflect the procedure of human wound healing better when compared with existing models.It can be considered as an ideal model for further study of wound healing. Key words: Wound healing; Model,animal; Silicone ring