Abstract
We propose and demonstrate a Twin-interferometry (TIM) based optical approach for studying and exploring anisotropy and directional dependent coagulation kinetics in human plasma under isothermal condition. Human plasma is obtained by centrifuging blood samples collected in Ethylenediamine tetra acetic acid (EDTA) coated vials at 6000 RPM in a laminar flow unit. 0.5 ml of plasma is collected in an optically flat disposable glass cuvette and subjected to induced coagulation by adding equal amounts of CaCl2 solution as coagulant at seven different concentrations. A Twin-interferometer is designed to record the rate of shift in Michelson’s fringes caused by rate of change of optical path difference of coagulating plasma in two orthogonal directions. A pair of interferograms, referred to as the Twin- interferogram (TIG), are recorded for all plasma samples with different CaCl2 concentration during its coagulation. A new approach for extracting various coagulation parameters from TIG, such as anisotropy, half coagulation time, constants governing the rate and dimensionality of coagulation, and their variation along distinct direction is demonstrated. TIG results are validated using polarizing optical microscopic (POM) and optical extinction techniques.
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