Abstract
Cigarette smoke is associated with several pathologies including chronic respiratory diseases and cancer. In addition, exposure to cigarette smoke is correlated with impaired wound healing, where a significant decrease in the regenerative capacity of smokers is well documented and broadly considered a negative risk factor after trauma or surgery. So far, some in vitro and in vivo models have been described to study how exposure to cigarette smoke diminishes the regenerative potential in different organisms. However, although useful, many of these models are difficult and expensive to implement and do not allow high-throughput screening approaches. In order to establish a reliable and accessible model, we have evaluated the effects of cigarette smoke extract (CSE) on zebrafish development and regeneration. In this work, zebrafish embryos and larvae were exposed to low doses of aqueous CSE showing severe developmental abnormalities in a dose-dependent manner. Furthermore, when adult zebrafish were subjected to caudal fin amputation, we observed a significant decrease in the regenerative capacity of animals exposed to CSE. The effect was exacerbated in male and aged fish compared to female or young organisms. The establishment of a zebrafish model to assess the consequences of cigarette smoke and its effects on animal physiology could provide a new tool to study the underlying mechanisms involved in impaired tissue regeneration, and aid the development of novel approaches to treat complications associated with cigarette smoke toxicity.
Highlights
Considered to be key for optimal health care delivery for patients with critical limb ischemia[13] and clinical procedures involving the musculoskeletal system in general[14]
These models have been used to study the effect of exposure to cigarette total particulate matter (TPM) in skin wound healing[27], and to address the damage induced by cigarette smoke on the olfactory epithelium, the impairment of nasal mucosa healing and the recovery of olfactory receptor neurons[28,29]
In order to evaluate whether zebrafish represents a viable model to study the toxicity of aqueous cigarette smoke extracts (CSE), 3–5 hpf embryos were incubated for 96 hours with different concentrations of CSE
Summary
Considered to be key for optimal health care delivery for patients with critical limb ischemia[13] and clinical procedures involving the musculoskeletal system in general[14]. On the other hand, have been reported to replicate the damage in bone integrity and the compromised bone regeneration outcome in tobacco consumers as well as in people exposed to secondhand smoke[24,25,26] These models have been used to study the effect of exposure to cigarette total particulate matter (TPM) in skin wound healing[27], and to address the damage induced by cigarette smoke on the olfactory epithelium, the impairment of nasal mucosa healing and the recovery of olfactory receptor neurons[28,29]. We decided to evaluate whether the zebrafish model could be established to study the toxic effects of fresh whole cigarette smoke extracts during embryonic and larval development, as well as a model for smoke-dependent impaired tissue regeneration in adult tissues
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
