Effects of cigarette smoke extract on the expression of SIRT6 and Caspase-9 in human bronchial epithelial cells

Abstract

Objective To observe the effect of cigarette smoke extract (CSE) on the expression of human silencing information regulator protein 6 (SIRT6) and Caspase-9 in human bronchial epithelial cells (16HBE). Methods In vitro culture of 16HBE, we added different concentrations of fresh CSE to induce cells.The expression of SIRT6 and Caspase-9 were detected by fluorescence quantitative polmerase chain reaction (PCR) and Western blotting.The effects of (0%, 5%, 7.5%, 10%)CSE on the expression of SIRT6 and Caspase-9 in 16HBE were compared. Results After 16HBE treatment with different concentrations (0%, 5%, 7.5%, 10%) of CSE, the SIRT6 mRNA expression showed significant difference (P 0.05). Caspase-9 mRNA expression between groups showed significant difference (P<0.01). The differences between each two groups were statistically significant (P<0.01). After 16HBE treatment with different concentrations (0%, 5%, 7.5%, 10%) of CSE, the SIRT6 protein expression and Caspase-9 protein showed significant difference between groups (P<0.01). The differences between each two groups were statistically significant (P<0.01 or<0.05). Conclusions CSE can cause SIRT6, Caspase-9 of 16HBE expression changes. Key words: Cigarette smoke extract; Human bronchial epithelial cells; Human silencing information regulator protein; Caspase-9