A novel in situ system to evaluate the effect of high CO2 on photosynthesis and biochemistry of seaweeds

Abstract

AB Aquatic Biology Contact the journal Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections AB 22:245-259 (2014) - DOI: https://doi.org/10.3354/ab00594 Theme Section: Environmental forcing of aquatic primary productivity A novel in situ system to evaluate the effect of high CO2 on photosynthesis and biochemistry of seaweeds N. Korbee1,*, N. P. Navarro2, M. García-Sánchez3, P. S. M. Celis-Plá1, E. Quintano4, M. S. Copertino5, A. Pedersen6, R. Mariath7, N. Mangaiyarkarasi8, Á. Pérez-Ruzafa3, F. L. Figueroa1, B. Martínez9 1Department of Ecology, Faculty of Science, University of Málaga, Campus de Teatinos s/n, 29071 Málaga, Spain 2Faculty of Science, University of Magallanes, Avenida Bulnes 01855 (casilla 113-D), Punta Arenas, Chile 3Department of Ecology and Hydrology, Faculty of Biology, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, Spain 4Department of Plant Biology and Ecology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), PO Box 644, 48080 Bilbao, Spain 5Institute of Oceanography, Federal University of Rio Grande-FURG, CP 474, cep 93206-900 Rio Grande (RS), Brazil 6Norwegian Institute for Water Research, Department of Marine Biology, Gaustadalléen 21, 0349 Oslo, Norway 7Department of Botany, Institute of Biology, Federal University of Rio de Janeiro, Brazil 8Plant Biology and Biotechnology, CKN College Thiruvalluvar University, Cuddalore, India 9Biology and Geology Department, Rey Juan Carlos University, Tulipán s/n, 28933 Móstoles, Spain *Corresponding author: nkorbee@uma.es ABSTRACT: Previous studies of the impact of increased CO2 on macroalgae have mainly been done in laboratories or mesocosm systems, placing organisms under both artificial light and seawater conditions. In this study, macroalgae were incubated in situ in UV-transparent cylinders under conditions similar to the external environment. This system was tested in a short-term study (5.5 h incubation) on the effect of 2 partial pressures of CO2 ( pCO2): air (ambient CO2) and the pCO2 predicted by the end of the 21st century (700 µatm, high CO2), on photosynthesis, photosynthetic pigments and photoprotection in calcifying (Ellisolandia elongata and Padina pavonica) and non-calcifying (Cystoseira tamariscifolia) macroalgae. The calcifying P. pavonica showed higher net photosynthesis under high CO2 than under ambient CO2 conditions, whereas the opposite occurred in C. tamariscifolia. Both brown algae (P. pavonica and C. tamariscifolia) showed activation of non-photochemical quenching mechanisms under high CO2 conditions. However, in P. pavonica the phenol content was reduced after CO2 enrichment. In contrast to phenols, in E. elongata other photoprotectors such as zeaxanthin and palythine (mycosporine-like amino acid) tended to increase in the high CO2 treatment. The different responses of these species to elevated pCO2 may be due to anatomical and physiological differences and could represent a shift in their relative dominance as key species in the face of ocean acidification (OA). More in situ studies could be carried out to evaluate how macroalgae will respond to increases in pCO2 in a future OA scenario. The in situ incubator system proposed in this work may contribute towards increasing this knowledge. KEY WORDS: Macroalgae · Non-photochemical quenching · Ocean acidification · Photoprotection · Photosynthesis Full text in pdf format PreviousNextCite this article as: Korbee N, Navarro NP, García-Sánchez M, Celis-Plá PSM and others (2014) A novel in situ system to evaluate the effect of high CO2 on photosynthesis and biochemistry of seaweeds. Aquat Biol 22:245-259. https://doi.org/10.3354/ab00594 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AB Vol. 22. Online publication date: November 20, 2014 Print ISSN: 1864-7782; Online ISSN: 1864-7790 Copyright © 2014 Inter-Research.