大气CO2升高和紫外辐射相互作用对羊栖菜生理特性的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 大气CO2升高和紫外辐射相互作用对羊栖菜生理特性的影响 DOI: 10.5846/stxb201305261184 作者: 作者单位: 淮海工学院海洋学院,淮海工学院海洋学院,华南理工大学环境与能源学院 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金(41276148, 41106093); 江苏省海洋生物技术重点实验室课题(2010HS08); 江苏省"青蓝工程"人才基金; 江苏高校优势学科建设工程资助项目 Combined effects of increasing CO2 concentrations and solar UV radiation on the physiological performance of Hizikia fusiformis Okamura (Sargassaceae, phaeophyta) Author: Affiliation: School of Marine Science ＆ Technology, Huaihai Institute of Technology,,College of Environment and Energy , South China University of Technology, Guangzhou 510006, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为了研究经济海藻羊栖菜对大气CO2浓度增加与紫外辐射(UVR)相互作用的响应,设置两个CO2浓度(380μL/L和800μL/L)以及两种辐射处理,即PAR处理(滤除UV-A、UV-B, 藻体仅接受可见光,400-700nm)和PAB处理(全波长辐射280-700nm)培养海藻,探讨了羊栖菜生长、光合作用、呼吸作用、光合色素含量、可溶性糖和蛋白以及硝酸还原酶活性的变化情况。结果表明高浓度CO2显著提高羊栖菜藻体的相对生长速率,并且紫外辐射的负面效应在高CO2处理下表现不显著。高CO2降低了藻体的光合作用速率,而UVR的负面效应和生长体现为一致性,但是羊栖菜的呼吸作用没有受到环境变化的明显影响。羊栖菜的光合色素叶绿素a和类胡萝卜素在高浓度CO2处理下明显降低,而UVR没有明显影响。环境因子对羊栖菜的可溶性糖没有影响,但是在高CO2和全波长辐射处理下,藻体可溶性蛋白的含量显著增加。同时高CO2明显提高了硝酸还原酶的活性,并且仅在高浓度CO2处理下藻体中UVR对其活性有抑制作用。CO2和UVR对羊栖菜的大多数生理特性存在明显的交互作用,在未来CO2浓度进一步增加的情况下,UVR的负面效应将会得到一定程度的缓解,这样有利于羊栖菜在养殖海区获得更高的产量。 Abstract:Here, we investigated the physiological responses of the economically important brown macroalgal species, Hizikia fusiformis, to solar ultra-violet (UV) radiation and increasing CO2 concentrations. Specifically, we cultured the alga under two different CO2 concentrations (380μL/L and 800μL/L) and two different solar radiations (PAR: photosynthetically active radiation 400-700 nm; PAB: full spectrum solar radiation, 280-700 nm) outdoors. Two radiation treatments were established in the experiment: (1) thalli that received full spectrum solar radiation (PAB treatment) in quartz tubes covered with Ultraphan 295, and (2) thalli that only received PAR (P treatment) in quartz tubes covered with Ultraphan film 395. CO2 was supplied to the different treatments by bubbling (300 mL/min) ambient air (390μL/L) or air enriched with CO2 (800μL/L CO2 in the air) with a plant CO2 incubator that automatically controlled CO2 concentrations, with less than 5% variation. For all treatments, algae were grown at 20℃, which was regulated by a cooling unit. The growth rate, photosynthesis, dark respiration, photosynthetic pigment contents, soluble carbohydrates, proteins, and nitrate reductase activity were measured after three weeks of culture. Our results showed that elevated CO2 significantly enhanced the relative growth rate of H. fusiformis. UV radiation appeared to have no negative effects on thalli grown under high CO2 conditions. High CO2 and UV culture conditions both reduced the net photosynthetic rate of H. fusiformis. However, no significant difference was found in the dark respiration of H. fusiformis among all treatments. Chlorophyll a and carotenoid contents declined when H. fusiformis was cultured under high CO2 conditions, whereas UV had no significant effect on the content of the two pigments. There was no difference in soluble carbohydrates among all treatments; however, compared to all other treatments, soluble protein content significantly increased in thalli grown at high CO2 and full spectrum solar radiation. Nitrate reductase activity was enhanced by high CO2 treatment, but was inhibited in thalli grown under both UV and high CO2 conditions. Overall, the physiological performance of H. fusiformis was most significantly influenced by CO2 and UVR, with increasing CO2 concentrations potentially alleviating the negative effects of UVR on thalli performance. This information is expected to help enhance the production of H. fusiformis grown in sea areas designated for future culturing. 参考文献 相似文献 引证文献