Abstract
Accumulated studies revealed that CD4 +T cells were initially required for diabetes in NOD mice, whereas interaction of CD4 +T/CD8 +T cells is not fully understood. To address this question, we established islet-derived CD4 +T cells and CD8 +T cells from NOD mice. One NOD neonate that received CD4 +T cells developed diabetes and insulitis with CD8 +T cells. Administration of cyclophosphamide to non-diabetic recipients accelerated the development of diabetes, while none of the mice with anti-CD8 antibody did so. Similarly, it was observed that neonates that received islet-derived CD8 +T cells developed diabetes and obvious insulitis mainly with CD4 +T cells. Administration of anti-CD4 antibody with transfer of CD8 +T cells inhibited insulitis. These results imply that CD8 +T cells function as an initial element to recruit CD4 +T cells to islets as well as a final effector.
Published Version
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