A novel fluorescent sensor for selective rifampicin detection based on the bio-inspired molecularly imprinted polymer-AgInS2/ZnS quantum dots.

Abstract

A fluorescent sensing material based on the ternary core-shell quantum dots with outstanding optical properties and a bio-inspired molecularly imprinted polymer (MIP) as a recognition element has been prepared for selective detection of rifampicin (RFP). Firstly, AgInS2/ZnS core/shell quantum dots (ZAIS QDs) were prepared by a hydrothermal process. Then, the fluorescent sensor was prepared by coating these QDs by a dopamine-based MIP layer. The fluorescence of MIP@ZAIS QDs was quenched by RFP probably due to the photoinduced electron transfer process. The quenching constant was much higher for MIP@ZAIS QDs than the non-imprinted polymer@QDs, indicating that MIP@ZAIS QDs could selectively recognize RFP. Under the optimized conditions, the sensor had a good linear relationship at the RFP concentration range of 5.0 to 300nM and the limit of detection was 1.25nM. The respond time of the MIP@ZAIS QDs was 5min, and the imprinting factor was 6.3. It also showed good recoveries ranging from 98 to 101%, for analysis of human plasma samples. The method is simple and effective for the detection of RFP and offers a practical application for the rapid analysis of human plasma samples.