A Novel Fluorescent Reagent for Analysis of Hydrogen Peroxide

Abstract

Abstract8‐(4,6‐Dichloro‐1,3,5‐triazinoxy)quinoline (DTQ) was evaluated as a new fluorescent reagent for determining hydrogen peroxide. It was found that the fluorescence intensity of DTQ in alkaline medium could be dramatically enhanced upon addition of H2Q2. Based on this effect, a simple and selective method for the spectrofluorimetric determination of hydrogen peroxide was established. The relative standard deviation of the method was found to be 1.1% for 9 replicate determinations of a 4.6 × 10–6 mol/L hydrogen peroxide solution. The linear range was 2.3 × 10–7—2.3 × 10–5 mol/L with a detection limit of 2.2 × 10–8 mol/L (S/N = 3). The method was attempted to determine hydrogen peroxide in synthetic human serum samples with satisfactory results.