A new flow cell design for chemiluminescence detection using an improved signal transduction network. Determination of hydrogen peroxide in pharmaceuticals

Abstract

In this study, it proposed for the first time, a setup consisting of a flow cell shaped like a hollow coin for chemiluminescence detection and a luminometer consisting of two photodiodes assembled with a new configuration. The feasibility of the proposed luminometer for signal transduction was ascertained using a light-emitting diode (LED) to simulate chemiluminescence emissions. The feasibility for analytical purposes was evaluated using the oxidizing reaction of luminol with hydrogen peroxide catalyzed by hexacyanoferrate(III) inion in alkaline medium. The system that handles sample and reagent solutions is based on the multicommuted flow analysis (MCFA) approach. It uses solenoid mini-pumps and peristaltic mini-pumps controlled by an Arduino Due board for fluid propulsion. After optimizing the operational conditions, peroxide samples (pharmaceutical formulation) were analyzed. The accuracy was assessed by comparing the results with those obtained using a reference method. The results of a paired t-test at the 95% confidence level demonstrate no significant difference between the results. Other favorable characteristics of our method include: a linear response between 1 and 100 μmol L−1 hydrogen peroxide (r2 = 0.999); limit of detection and limit of quantification of 0.13 and 0.43 µmol L−1 H2O2, respectively; luminol and hexacyanoferrate(III) inion consumption of 60 µg (0.3 µmol L−1) and 1.9 mg (6 µmol L−1) per determination, respectively; a sampling rate of 230 determinations per hour; waste generation of 0.63 mL per determination; and a coefficient variation of 1.5% (n = 11) for a 0.17 mg L−1 (5 µmol L−1) hydrogen peroxide solution.