A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus

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In this artice, we are going to report results of a study in which with the help of layer-by-layer modification of a glassy carbon electrode (GCE), a novel biosensor was fabricated whose impedimetric and amperometric responses were correlated with the number of pneumococcus (P). The GCE was modified by graphene-multiwalled carbon nanotubes (Gr-MWCNTs)/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (CS-IL)/gold‑lead nanoparticles (AuPb NPs)/DNA-[FAM]CGC AAT CTA GCA GAT GAA GCA GGA AAA AAA AAA [Thiol] (DNA-Q-S). All the modification layers were characterized by microscopic and electrochemical techniques which confirmed the successfulness of all of the modification steps. The charge transfer resistance of impedimetric response and current of amperometric response of the biosensor were correlated with the number of the Ps which helped us to develop a novel biosensing system for sensing the Ps whose performance was comparable with a reference method. Therefore, the biosensor can be suggested for clinical purposes as a fast, stable, simple, sensitive, selective, repeatable and reproducible electronic device.