A novel continuous arterial spin labeling approach for CBF measurement in rats with reduced labeling time and optimized signal-to-noise ratio efficiency

Abstract

To develop a continuous arterial spin labeling (CASL) perfusion imaging method for cerebral blood flow (CBF) measurement in rats with reduced spin-labeling length and optimized signal-to-noise ratio (SNR(f)) per unit time. In the proposed method, the longitudinal magnetization of brain tissue water in the imaging slice is prepared into a proper state before spin-labeling, and a post-tagging delay is employed after spin-labeling. The method was implemented on a 4.7 T small animal scanner. Numerical simulations and in vivo experiments were used to evaluate the performance of the method proposed. With the proposed method, absolute CBF could be measured accurately from normal rat with a spin-labeling pulse as short as 400 ms, and yet employing the same formula as that used in the conventional CASL perfusion imaging method for calculation. The method also showed improved SNR(f) per unit time over the conventional CASL perfusion imaging method and the pulsed arterial spin labeling perfusion imaging method FAIR. Compared to the conventional CASL perfusion imaging method, the proposed method would be advantageous for CBF measurement in small animals having short vascular transit time in terms of SNR(f) per unit time and other benefits brought by shortened spin-labeling pulse.