A novel conserved mechanism for plant NLR protein pairs: the "integrated decoy" hypothesis.

Thomas Kroj,Peter N. Dodds,Stella Cesari,Maud Bernoux,Philippe Moncuquet

doi:10.3389/fpls.2014.00606

Abstract

Plant immunity is often triggered by the specific recognition of pathogen effectors by intracellular nucleotide-binding, leucine-rich repeat receptors (NLR). Plant NLRs contain an N-terminal signaling domain that is mostly represented by either a Toll-interleukin1 receptor (TIR) domain or a coiled coil (CC) domain. In many cases, single NLR proteins are sufficient for both effector recognition and signaling activation. However, many paired NLRs have now been identified where both proteins are required to confer resistance to pathogens. Recent detailed studies on the Arabidopsis thaliana TIR-NLR pair RRS1 and RPS4 and on the rice CC-NLR pair RGA4 and RGA5 have revealed for the first time how such protein pairs function together. In both cases, the paired partners interact physically to form a hetero-complex receptor in which each partner plays distinct roles in effector recognition or signaling activation, highlighting a conserved mode of action of NLR pairs across both monocotyledonous and dicotyledonous plants. We also describe an “integrated decoy” model for the function of these receptor complexes. In this model, a plant protein targeted by an effector has been duplicated and fused to one member of the NLR pair, where it acts as a bait to trigger defense signaling by the second NLR upon effector binding. This mechanism may be common to many other plant NLR pairs.

Highlights

Plants, unlike animals do not possess circulating immune cells to intercept microbial signals
Distinct nucleotidebinding and leucine-rich repeat receptors (NLR) proteins: (i) form homo- and hetero-complexes that are involved in important regulation processes both prior to and following pathogen recognition, (ii) cooperate to achieve one task, but are specialized and accomplish distinct functions within the complex (i.e., avirulence proteins (AVR)-receptor or signal inducer), (iii) recognize multiple AVR proteins through direct binding to the “receptor” partner which possesses an unusual domain that is not conserved in other NLRs, (iv) rely on the N-terminal Toll/interleukin-1 receptor (TIR) or CC domain of the “signal inducer” NLR to translate recognition into activation of resistance responses
Despite high sequence similarity in the CC-NLR domains, the RATX1 domain is not conserved among RGA5 rice homologs, nor in a wider set of RGA5 homologs identified in cereals, and this C-terminal region is substituted in many cases by different protein domains that may act as integrated decoys (Table 2, Supplemental Tables 1, 2)

Summary

Introduction

Unlike animals do not possess circulating immune cells to intercept microbial signals. The paired partners interact physically to form a hetero-complex receptor in which each partner plays distinct roles in effector recognition or signaling activation, highlighting a conserved mode of action of NLR pairs across both monocotyledonous and dicotyledonous plants.

Results

Conclusion