Abstract
A 1,8-naphthalimide hydrazone derivative bearing a pyrrole unit is a highly selective ratiometric fluorescent Cu2+ probe used to monitor intracellular Cu2+ in HeLa cells. This probe functions via a unique hydrolysis mechanism through Cu2+-promoted electrophilic substitution. Binding is confirmed through UV–vis absorption, fluorescence measurements, mass spectroscopy, IR, X-ray single crystal diffraction, and DFT calculation.
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