Abstract
A novel chemical profile essential oil, distilled from the aerial parts of Clinopodium taxifolium (Kunth) Govaerts (Lamiaceae), was analysed by Gas Chromatography-Mass Spectrometry (GC-MS, qualitative analysis) and Gas Chromatography with Flame Ionization Detector (GC-FID, quantitative analysis), with both polar and non-polar stationary phase columns. The chemical composition mostly consisted of sesquiterpenes and sesquiterpenoids (>70%), the main ones being (E)-β-caryophyllene (17.8%), α-copaene (10.5%), β-bourbonene (9.9%), δ-cadinene (6.6%), cis-cadina-1(6),4-diene (6.4%) and germacrene D (4.9%), with the non-polar column. The essential oil was then submitted to enantioselective GC analysis, with a diethyl-tert-butyldimethylsilyl-β-cyclodextrin diluted in PS-086 chiral selector, resulting in the following enantiomeric excesses for the chiral components: (1R,5S)-(−)-α-thujene (67.8%), (1R,5R)-(+)-α-pinene (85.5%), (1S,5S)-(−)-β-pinene (90.0%), (1S,5S)-(−)-sabinene (12.3%), (S)-(−)-limonene (88.1%), (S)-(+)-linalool (32.7%), (R)-(−)-terpinen-4-ol (9.3%), (S)-(−)-α-terpineol (71.2%) and (S)-(−)-germacrene D (89.0%). The inhibition activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) of C. taxifolium essential oil was then tested, resulting in selective activity against BChE with an IC50 value of 31.3 ± 3.0 μg/mL (positive control: donepezil, IC50 = 3.6 μg/mL).
Highlights
Publisher’s Note: MDPI stays neu-According to the UN Environment Program World Conservation Monitoring Central with regard to jurisdictional claims tre [1], Ecuador belongs to the group of the 17 megadiverse countries in the world, making in published maps and institutional it an extraordinary reservoir of biodiversity
Most of the detected constituents were identified according to the corresponding Electron Ionization Mass Spectrum (EIMS) and Linear Retention Index (LRI)
The C. taxifolium essential oils (EOs) produced a selective inhibition of BChE, differing from what is usually described in the literature, where (E)-β-caryophyllene, and some of the above mentioned terpenoids, inhibit both enzymes [41]
Summary
Publisher’s Note: MDPI stays neu-According to the UN Environment Program World Conservation Monitoring Central with regard to jurisdictional claims tre [1], Ecuador belongs to the group of the 17 megadiverse countries in the world, making in published maps and institutional it an extraordinary reservoir of biodiversity. In 2016, two of us (J.R. and G.G.) reported that about 50% of all the phytochemical publications on the Ecuadorian flora referred only to 8 of the 245 botanical families of the native species known in the country [2]. For this reason, the authors have been involved for years in the description of specialized (secondary) metabolites contained in the Ecuadorian flora, to contribute to the advance in its phytolicense Within a project focused on the licenses/by/4.0/)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
