Abstract
Bovine and ovine lutropins (bLH and oLH) have three similar asparagine-linked carbohydrate units made up of Fuc, Gal (present only in oLH), Man, GlcNAc and GalNAc. The structural analyses of these carbohydrate units were performed on the oligosaccharides obtained by the alkaline borohydride treatment of the hormones and on the native hormones. The determination of intersugar and anomeric linkages, monosaccharide sequences and the polypeptide-carbohydrate linkage was carried out by methylation, periodate oxidation and deamination techniques and treatment with exoglycosidases. Based on these studies the structure for the oligosaccharide of bLH and oLH is proposed.
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