Abstract
The detailed structures of the carbohydrate moiety of hCG have been determined. The structural analyses were carried out on all four asparagine-linked glycopeptides as well as serine-linked carbohydrate chains. The glycopeptides were prepared from the tryptic hydrolysates of the reduced-S-carboxamidomethyl hCG-α and hCG-β and were purified by chromatography on Sephadex G-50 and preparative high voltage paper electrophoresis at pH 1.8. The serine-linked carbohydrate chains were cleaved by β-elimination with alkali in the presence of sodium borohydride and were purified by chromatography on Sephadex G-25. All glycopeptides and the oligosaccharide were examined for homogeneity by high voltage paper electrophoresis, paper chromatography, and chemical composition. The structural studies involved the determination of intersugar and anomeric linkages and monosaccharide sequences and were carried out by a combination of several techniques such as periodate oxidation, methylation and sequential enzymatic degradation.
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