Abstract
SEREX has proven to be a powerful method that takes advantage of the presence of spontaneous humoral immune response in some cancer patients. In this study, immunoscreening of normal testis and two ovarian cancer cell line cDNA expression libraries with sera from ovarian cancer patients led to the isolation of 75 independent antigens, designated KP-OVA-1 through KP-OVA-75. Of these, RT-PCR showed KP-OVA-52 to be expressed strongly in normal testis, in ovarian cancer cell lines (3/9) and in ovarian cancer tissues (1/17). The expression of KP-OVA-52 in cancer cells is also induced by the demethylating agent 5-aza-2′-deoxycytidine (ADC). To test immunogenicity, we used the Serum Antibody Detection Assay (SADA) to analyze anti-IgG antibodies against the 75 antigens that were initially isolated by SEREX. Four of the 75 antigens (KP-OVA-25, KP-OVA-35, KP-OVA-68 and KP-OVA-73) reacted exclusively with sera from cancer patients. However, KP-OVA-52 reacted with 1 of 20 ovarian cancer sera. These data suggest that the KP-OVA-52 can be considered a novel CT antigen that is regulated by DNA methylation.
Highlights
Ovarian cancer is the leading cause of death from gynaecological malignancies [1,2]
These 75 antigens comprise of 66 known proteins together with 9 uncharacterized gene products, which does not present in the sequences designated in the databases as expressed sequence tags (ESTs), KIAA series clones, FLJ series clones, MGC series clones, DKFZ series clones, and anonymous open reading frames (ORFs)
serologic analysis of recombinant cDNA expression libraries (SEREX) methodology was applied to further definine the spectrum of immunogenic proteins in serous ovarian cancer patients
Summary
Ovarian cancer is the leading cause of death from gynaecological malignancies [1,2]. It accounts for 5% of all cancer deaths among women with an estimated 21,880 new cases and 13,850 deaths from ovarian cancer in the United States in 2010 [2]. Because so many ovarian cancer patients are diagnosed at a later stage, it is important to find methods by which to improve treatments for more advanced disease
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