Abstract
Reliable and precise methods are to be applied ranging from biological field to diagnosis and quality control to identify target analytes in samples. Nuclear magnetic resonance (NMR) is a strongly recommended process that is suitable for collecting data multidimensionally at molecular and atomic levels. Information obtained from the NMR technique provides molecular structures, molecular interactions, dynamic and diffusional properties of molecules. Overlapping of NMR signals of each component makes it difficult to interpret data and low sensitivity makes it unfitting for many samples with a low concentration of analytes. These limitations can be rectified by adding heteronuclear specially designed sensors, inventive transduction mechanism and strong pulse sequences. 19F NMR tool is extensively accepted method to detect the interactions among a variety of biological substrates with 19F-isotopic species. This review includes the detection of biomolecules, mainly enzymes and enzymatic reactions based on sensor applicability.
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