A novel α-fetoprotein-MIP immunosensor based on AuNPs/PTh modified glass carbon electrode

Abstract

A new α-fetoprotein-MIP (AFP-MIP) immunosensor based on glass carbon electrode (GCE) modified with polythionine (PTh) and gold nanoparticles (AuNPs) was successfully fabricated for sensitive detection of α-fetoprotein (AFP). Through controlling electropolymerization, A “polydopamine (PDA)-AFP” complex was achieved applying AFP as template and dopamine (DA) as imprinted monomers. After elution, the specific cavities can adsorb the target molecules. Using differential pulse voltammetry (DPV) detection, the peak current decreased with the increase in concentration of AFP, and the linear response range of the AFP-MIP immunosensor was from 0.001 ng/mL to 800 ng/mL with the detection limit as low as 0.8138 pg/mL. The MIP immunosensor could become a new promising method for the detection of AFP. Furthermore, this MIP sensor was demonstrated in testing AFP in human serum samples with satisfactory results.