Abstract
Non-covalent complex of lipid-coated CdSe/ZnS quantum dots and second-generation photosensitizer, chlorin e6 can enter living HeLa cells with maintained integrity that ensures efficient FRET.
Highlights
IntroductionPaper interface between water and the quantum dots (QD) coating, which is relatively far from the QD core
The unique optical properties of semiconductor quantum dots (QD) as well as their nano-dimensions, stability and ease of surface modi cation make these nanoparticles attractive for many biological and medical applications.[1,2,3,4,5,6,7,8] In 2003 Samia et al suggested the exploitation of QD as resonance energy donors for classical photosensitizers (PS) used in the photodynamic therapy (PDT) of cancer.[1]
Spectroscopic changes, the highly efficient Forster resonance energy transfer (FRET), observed upon chlorin e6 (Ce6) binding to QD, and remarkable stability of the QD–Ce6 complex in different media suggest that Ce6 penetrates inside the lipid coating close to the inorganic core of QD
Summary
Paper interface between water and the QD coating, which is relatively far from the QD core. Despite the numerous studies on different QD–PS systems in aqueous solutions, there are only a few reports on stability and FRET properties of QD–PS systems studied in vitro.[26,27,28]. We prepared complexes of commercial CdSe/ ZnS QD bearing a lipid-based coating with different terminal groups (carboxyl, amine and non-functionalized) with chlorin e6 (Ce6), a well-known second-generation photosensitizer having a high quantum yield of singlet oxygen production (Scheme 1).[29] We obtained exceptionally high FRET efficiency of these complexes, suggesting that Ce6 is rmly imbedded inside QD lipid coating close to the inorganic core. Most importantly, according to the uorescence lifetime imaging (FLIM) with twophoton excitation, these QD–Ce6 complexes readily entered living HeLa cells with maintained efficient FRET, which shows their remarkable stability in the intracellular media
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